The Proteomics Research and Services Unit (jointly owned by the ARC and UWC)-, provides State-of‐the‐Art Proteomics research and services, mostly to Agricultural and Biotechnology researchers, as well as to private companies in South Africa. This work is mostly funded by the Department of Science and Technology (DST) through the National Equipment Programme (NEP) of the National Research Foundation (NRF).
Proteomics, generally defined as the simultaneous and high throughput study of protein expression profiles, is used to monitor proteome profiles towards the understanding of molecular mechanisms associated certain biological and environmental cues.
The MALDI Biotyper enables molecular identification, and classification of microorganisms like bacteria, yeasts and fungi. This application is achieved reliably and quickly using proteomic finger-printing by high-throughput MAL-DI-TOF mass spectrometry.
Some of our tools:Polymer AnalysisProtein Gel Imaging nanoHPLC1D/2D SDS PAGEMALDI Imaging Protein IdentificationProtein ExtractionRobotic Spot Picking Metabolite AnalysisMALDI Bio-typing (Micro-organism Identification)Protein Separation
Some of our tools:
Protein Gel Imaging
1D/2D SDS PAGE
Robotic Spot Picking
MALDI Bio-typing (Micro-organism Identification)
Identification and profiling of abiotic stress-responsive proteins in Arabidopsis thaliana and Sorghum bicolor.
Analysis of sugar biosynthesis-related proteins of sorghum bicolor and investigation of their role in drought-stress tolerance
Evaluation of the suitability and sustainability of Sweet Sorghum for bioethanol (and other by-products) production in South Africa
Guayule Proteome Analysis: Towards development of a sustainable low tech-hypoallergenic latex (and by products) agroprocessing system designed for South African small-holder farmers.
A number of fee-for-service and cost sharing projects are on-going.
Example of a Bio-Typing process
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