The ARC-BTP has implemented high-throughput DNA and RNA sequencing and genotyping technologies within the platform, with the capacity to genotype thousands of samples, or generates large volumes of data from single samples.

The current technologies available in the platform include:

  • Next generation sequencing and genotyping platforms

  • Liquid handling robotics systems

  • Laser capture micro-dissection microscope​

Next Generation Sequencing
SNP Genotyping
High throughput nucleic acid extraction


BTP Core Staff Members

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Dr Minique de Castro
Application Specialist: Next Generation Sequencing

E-mail: 
decastrom@arc.agric.za
Phone: +27 (0)12 529 9483
External links
: Google Scholar link

Dr de Castro holds a PhD degree in Life Sciences at the University of South Africa. At the Genomics Core Facility, she is responsible for library preparation and sequencing using Next Generation Sequencing technologies.

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Nompilo Hlongwane
Application Specialist: Genotyping and Next Generation Sequencing
E-mail: HlongwaneN@arc.agric.za
Phone: +27 (0)12 529 9482

Nompilo Hlongwane (MSc) is an Application Specialist: Genotyping in the ARC-CORE. Ms Hlongwane conducts genome-wide SNP genotyping within the Biotechnology Platform for the provision of services within research programs. Nompilo is currently pursuing a PhD in Genetics at the University of Kwa-Zulu Natal. Her research focus is genetic diversity, population genomics and signatures of selection in indigenous pigs. Her training enables her to provide genotyping sample preparation and SNP BeadChip data analysis support using different bioinformatics workflows.

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Tondani TM. Ramantswana
Application Specialist: Next Generation Sequencing
E-mail: 
RamantswanaT@arc.agric.za
Phone: +27 (0)12 529 9483

Madeleine has an MSc in Zoology and is an Application Specialist: Next Generation Sequencing. She is responsible for library preparation and sequencing using Next Generation Sequencing technologies. is currently pursuing a PhD in Life Science focusing on RNA-Seq work with Sheep gut.

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Alister Ngobeni
Application Specialist: High throughput Technologies

E-mail:
Ngobenia@arc.agric.za
Phone: 
+27 (0)12 529 9483

Mr Ngobeni has an MSc Biochemistry degree from the University of Limpopo. He manages daily operations of liquid handling facility for the provision of services to research programs within the ARC and external clients. As part of the liquid handling facility he is responsible for protocols programming and optimization on the Hamilton liquid handlers.

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Nick NR. Mokotoane
Application Specialist: Genotyping and Next Generation Sequencing

E-mail: MokotoaneN@arc.agric.za
Phone: +27 (0)12 529 9482

Mr Mokotoane holds a Bachelors and Honors degree in Molecular Biology and Genetics respectively, from the university of the Free State and has also obtained a research-based M.Sc. degree in Veterinary Science from the University of Pretoria.  Although his main focus within the Genomics Core Facility is genotyping he is also involved in the NGS workflow. 


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